Cleaning of Tomato brown rugose fruit virus (ToBRFV) from Contaminated Clothing of Greenhouse Employees

The highly infectious Tomato brown rugose fruit virus (ToBRFV) is a new viral threat to tomato production worldwide. In production, the very easy mechanical transmissibility combined with high resistance in vitro of great concern. We tested: (i) whether household cleaning products, commercial agricultural detergents, and an authorized plant protectant are suitable for contaminated clothing, (ii) viruses remain resulting water. evaluation sanitation effect was performed using bioassays, by counting ToBRFV-associated necrotic local lesions on Nicotiana tabacum cv. Xanthi NN. For this purpose, leaves were mechanically inoculated treated fabrics solutions which would normally be discharged sewer system. detergents Fadex H+ (FH) Menno Hortisept Clean Plus, as well disinfectant Florades (MF), led almost complete removal ToBRFV from fabrics, corresponding reduction 99.94–99.96%. contrast, common products (Spee ActivGel (SAG), Vanish Oxi Action Gel (VO) did not effectively remove pathogen fabric, where 45.1% 89.7%, respectively. particular, after use cleaners ToBRFV. After 16-h treatment MF, no longer present VO, FH, MF solutions, demonstrated extensive bioassays.